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3.2 Release of UV-absorbing substances from cells treated by PEF The presence of ultraviolet-absorbing material such as nucleic acid, proteins, and adenosine triphosphate (ATP) in the medium in which microbial cells are suspended is a common technique applied with the purpose of showing that electroporation has rendered the cytoplasmic membrane permeable to intracellular material that would otherwise be unable to cross it (Figure 3 illustrates the release of substances absorbing at 260 and 280 nm from the biomass of S

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